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Registros recuperados : 24 | |
1. | | FRASÃO, B. S.; CÔRTES, L. R.; NASCIMENTO, E. R.; CUNHA, N. C.; ALMEIDA, V. L.; AQUINO, M. H. C. Detecção de resistência às fluoroquinolonas em Campylobacter isolados de frangos de criação orgânica. Pesquisa Veterinária Brasileira, Rio de Janeiro, v. 35, n. 7, p. 613-619. jul. 2015. Título em inglês: Detection of fluoroquinolone resistance in Campylobacter strains from organic poultry. Biblioteca(s): Embrapa Unidades Centrais. |
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3. | | MAIA, A. L. R. e S.; SOUZA-FABJAN, J. M. G. de; RANGEL, P. S. C.; CÔRTES, L. R.; OLIVEIRA, M. E. F.; FONSECA, J. F. da. Estrus induction in the non-breeding season is not associated with hydrometra in dairy goats. Research, Society and Development, v. 10, n. 11, e21101119162, 2021. Biblioteca(s): Embrapa Caprinos e Ovinos. |
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4. | | ARRAIS, A. M.; MELLO, M. R. B.; CORTÊS, L. R.; BONATO, G. C.; OLIVEIRA, T. A.; CARVALHO, B. P.; FONSECA, J. F. da. Administration of hCG on the seventh day after initiation of estrus may circumvent negative effects of cervical relaxation protocol in sheep: Preliminary results. Animal Reprodroduction, v. 15, n. 3, p. 388, Jul./Sept. 2018. Abstract A073. Edição dos Proceedings of the 32nd Annual Meeting of the Brazilian Embryo Technology Society (SBTE); Florianópolis, SC, Brazil, August 16th to 18th, 2018. Biblioteca(s): Embrapa Caprinos e Ovinos. |
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5. | | MAIA, A. L. R. S.; SILVA, M. R.; BRANDÃO, F. Z.; SOUZA-FABJAN, J. M. G.; FARIA, L. S.; CÔRTES, L. R.; FACO, O.; FONSECA, J. F. da. Epidemiological survey and risk factors associated with hydrometra in dairy goat herds. Small Ruminant Research, v. 178, p. 79-84, Sept. 2019. Biblioteca(s): Embrapa Caprinos e Ovinos; Embrapa Gado de Leite. |
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6. | | FONSECA, J. F. da; VERGANI, G. B.; CÔRTES, L. R.; RODRIGUES, J. N. D.; SIQUEIRA, L. G. B.; SOUZA-FABJAN, J. M.; OLIVEIRA, M. E. F. Induction of accessory corpus luteum in goats and sheep: from physiological basis to reproductive effects. Revista Brasileira de Reprodução Animal, v. 45, n. 4, p. 309-317, out./dez. 2021. Edição dos anais do XXIV Congresso Brasileiro de Reprodução Animal e VIII International Symposium on Animal Biology of Reproduction, Belo Horizonte, 2021. Biblioteca(s): Embrapa Gado de Leite. |
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7. | | FONSECA, J. F. da; VERGANI, G. B.; CÔRTES, L. R.; RODRIGUES, J. N. D.; SIQUEIRA, L. G. B.; SOUZA-FABJAN, J. M.; OLIVEIRA, M. E. F. Induction of accessory corpus luteum in goats and sheep: From physiological basis to reproductive effects. Revista Brasileira de Reprodução Animal, v. 45, n. 4, p. 309-317, out./dez. 2021. Anais do XXIV Congresso Brasileiro de Reprodução Animal (CBRA-2021) e VIII International Symposium on Animal Biology of Reproduction - Joint Meeting, Belo Horizonte, MG, 19 a 22 de outubro de 2021. Biblioteca(s): Embrapa Caprinos e Ovinos. |
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8. | | BRAGANÇA, G. M.; BATISTA, R. I. T. P.; FABJAN, J. M. G. de S.; ALFRADIQUE, V. A. P.; CÔRTES, L. R.; CAMARGO, L. S. de A.; FONSECA, J. F. da; BRANDÃO, F. Z. FSH dose and strategy of administration during ovarian simulation alter the gene expression profile in ovine cumulus-oocyte complexes. In: ANNUAL MEETING OF THE BRAZILIAN EMBRYO TECNNOLOGY SOCIETY, 14., 2017, Cabo de Santo Agostinho. Proceedings... Cabo de Santo Agostinho: SBTE, 2017. p. 746. Biblioteca(s): Embrapa Gado de Leite. |
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9. | | BRAGANÇA, G. M.; BATISTA, R. I. T. P.; SOUZA FABJAN, J. M. G. de; ALFRADIQUE, V. A. P.; CORTÊS, L. R.; CAMARGO, L. S. de A.; FONSECA, J. F. da; BRANDÃO, F. Z. FSH dose and strategy of administration during ovarian stimulation alter the gene expression profile in ovine cumulus-oocyte complexes. Animal Reprodroduction, v. 14, n. 3, p. 746, Jul./Sept. 2017. Proceedings of the 31st Annual Meeting of the Brazilian Embryo Technology Society (SBTE), Cabo de Santo Agostinho, PE, Brazil, August 17 to 19, 2017. Biblioteca(s): Embrapa Caprinos e Ovinos. |
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10. | | MACHADO, V. de O.; LEAL, F. S. C.; BONATO, G. C.; DIAS, J. H.; ARRAIS, A. M.; MAIA, A. L. R. e S.; CÔRTES, L. R.; SOUZA-FABJAN, J. M. G.; FONSECA, J. F. da. Administração de hCG após a inseminação artificial eleva a taxa de gestação em cabras Saanen acíclicas submetidas a indução de estro sincronizado. Revista Brasileira de Reprodução Animal, v. 43, n. 2, p. 545, abr./jun. 2019. Edição dos resumos do XXIII Congresso Brasileiro de Reprodução Animal, Gramado, RS, Brasil, 15 a 17 de maio 2019. Biblioteca(s): Embrapa Caprinos e Ovinos. |
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11. | | CORTÊS, L. R.; FRAGA, M. O.; SILVA, D. S.; PRATES, J. F.; MARTINS, B. B.; FIGUEIRA, L. M.; BONATO, G. C.; ARRAIS, A. M.; SOUZA-FABJAN, J. M. G.; BRANDÃO, F. Z.; FONSECA, J. F. da. Administration of HCG seven days after estrous onset increases the pregnancy rate in Toggenburg goats subjected to induction of synchronized estrus and natural mating. Animal Reprodroduction, v. 15, n. 3, p. 366, Jul./Sept. 2018. Abstract A051. Edição dos Proceedings of the 32nd Annual Meeting of the Brazilian Embryo Technology Society (SBTE); Florianópolis, SC, Brazil, August 16th to 18th, 2018. Biblioteca(s): Embrapa Caprinos e Ovinos. |
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12. | | CÔRTES, L. R.; SOUZA-FABJAN, J. M. G.; DIAS, D. S.; MARTINS, B. B.; MAIA, A. L. R. S.; VEIGA, M. O.; ARASHIRO, E. K. N.; BRANDÃO, F. Z.; OLIVEIRA, M. E. F.; BARTLEWSKI, P. M.; FONSECA, J. F. da. Administration of a single dose of 300 IU of human chorionic gonadotropin seven days after the onset of estrus improves pregnancy rate in dairy goats by an unknown mechanism. Domestic Animal Endocrinology, v. 74, e106579, 2021. Biblioteca(s): Embrapa Caprinos e Ovinos. |
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13. | | BRAGANÇA, G. M.; SOUZA-FABJANA, J. M. G.; RIBEIRO, L. S.; BRAIRA, V. L.; CÔRTES, L. R.; SOUZA, C. V.; BATISTA, R. I. T. P.; FONSECA, J. F. da; MENCHACA, A.; BRANDÃO, F. Z. Exogenous progestogen does not affectfirst-wave folliclepopulations and oocyte quality during ovarian stimulationwith FSH in sheep. Domestic Animal Endocrinology, v. 72, e106369, Jul. 2020. Online ahead of print. Biblioteca(s): Embrapa Caprinos e Ovinos. |
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14. | | DIAS, J. H.; CORTÊS, L. R.; BRANDÃO, F. Z.; BONATO, G. C.; SOUZA-FABJAN, J. M. G.; ARRAIS, A. M.; OLIVEIRA, T. A.; MARTINS, B. B.; FACO, O.; FONSECA, J. F. da. Intravaginal hCG administration increases pregnancy rate in artificially inseminated cyclic dairy goats subjected to estrous synchronization. Animal Reprodroduction, v. 15, n. 3, p. 364, Jul./Sept. 2018. Abstract A049. Edição dos Proceedings of the 32nd Annual Meeting of the Brazilian Embryo Technology Society (SBTE); Florianópolis, SC, Brazil, August 16th to 18th, 2018. Biblioteca(s): Embrapa Caprinos e Ovinos. |
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15. | | BRAGANÇA, G. M.; SOUZA-FABJAN, J. M. G.; BATISTA, R. I. T. P.; RIBEIRO, L. S.; BRAIR, V. L.; CÔRTES, L. R.; SOUZA, C. V.; TRAIRA, A. R.; FONSECA, J. F. da; MENCHACA, A.; BRANDÃO, F. Z. Different progestagens sources do not affect the follicular population and the morphological quality of oocytes during ovarian stimulation in Santa Inês ewes. Animal Reprodroduction, v. 15, n. 3, p. 451, Jul./Sept. 2018. Abstracts A151. Edição dos Proceedings of the 32nd Annual Meeting of the Brazilian Embryo Technology Society (SBTE); Florianópolis, SC, Brazil, August 16th to 18th, 2018. Biblioteca(s): Embrapa Caprinos e Ovinos. |
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16. | | BRAGANÇA, G. M.; ALFRADIQUE, V. A. P.; BATISTA, R. I. T. P.; SOUZA-FABJAN, J. M. G. de; ARASHIRO, E. K. N.; BALARO, M. F. A.; OLIVEIRA, I. C. de; BRANDÃO, F. Z.; CÔRTES, L. R.; FONSECA, J. F. da. Efeito de diferentes protocolos hormonais para superestimulação ovariana sobre o número e a qualidade de oócitos em ovelhas da raça Santa Inês. Revista Brasileira de Reprodução Animal, Belo Horizonte, v. 41, n. 1, p. 470, jan./mar. 2017. Edição dos anais do XXII Congresso Brasileiro de Reprodução Animal (CBRA), Santos, SP, Brasil, maio 2017. Biblioteca(s): Embrapa Caprinos e Ovinos. |
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17. | | COSENTINO, I. O.; BALARO, M. F. A.; LEAL, F. S. C.; BADE, A. L. C.; CÔRTES, L. R.; ARASHIRO, E. K. N.; FONSECA, J. F. da; BRANDÃO, F. Z. Color Doppler ultrasonography for early pregnancy diagnosis in goats. Animal Reproduction, v. 15, p. 1112. 2018. Suplemento 1. Abstract 098. Edição dos Proceedings of the 10th International Ruminant Reproduction Symposium (IRRS), Foz do Iguaçu, PR, Brazil, September 16th to 20th, 2018. Biblioteca(s): Embrapa Caprinos e Ovinos. |
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18. | | BONATO, G. C.; CÔRTES, L. R.; ARRAIS, A. M.; FIGUEIRA, L. M.; OLIVEIRA, T. de A.; MACHADO, V. de O.; MAIA, A. L. R. e S.; SOUZA-FABJAN, J. M. G.; FONSECA, J. F. da. Effects of two d-cloprostenol administrations given at different intervals on estrus and ovulation in cyclic dairy goats. Revista Brasileira de Reprodução Animal, v. 43, n. 2, p. 538, abr./jun. 2019. Edição dos resumos do XXIII Congresso Brasileiro de Reprodução Animal, Gramado, RS, Brasil, 15 a 17 de maio 2019. Biblioteca(s): Embrapa Caprinos e Ovinos. |
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19. | | BONATO, G. C.; MAIA, A. L. R. S.; CÔRTES, L. R.; OLIVEIRA, T. A.; ARRAIS, A. M.; FIGUEIRA, L. M.; OLIVEIRA, M. E. F.; SOUZA-FABJAN, J. M. G.; FONSECA, J. F. da. Effects of d-cloprostenol administrations with 7.5 and 11.5-day intervals between administrations on pregnancy rates after artificial insemination in estrous cyclic dairy goats. Animal Reproduction Science, v. 209, article 106172, Oct. 2019. Biblioteca(s): Embrapa Caprinos e Ovinos. |
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20. | | BONATO, C. G.; SOUZA-FABJAN, J. M. G.; CÔRTES, L. R.; OLIVEIRA, T. A.; ARRAIS, E. M.; MAIA, A. L. R. S.; PRATES, J. F.; VEIGA, M. O.; FONSECA, J. F. da. Synchronization of estrus with two doses of cloprostenol at different intervals and Artificial Insemination in dairy goats. Animal Reprodroduction, v. 15, n. 3, p. 360, Jul./Sept. 2018. Abstract A045. Edição dos Proceedings of the 32nd Annual Meeting of the Brazilian Embryo Technology Society (SBTE); Florianópolis, SC, Brazil, August 16th to 18th, 2018. Biblioteca(s): Embrapa Caprinos e Ovinos. |
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Registros recuperados : 24 | |
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Registro Completo
Biblioteca(s): |
Embrapa Caprinos e Ovinos. |
Data corrente: |
22/01/2020 |
Data da última atualização: |
23/01/2020 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Circulação/Nível: |
B - 1 |
Autoria: |
ALFRADIQUE, V. A. P.; OLIVARES, C. C. S.; SOUZA-FABJAN, J. M. G. de; FONSECA, J. F. da; BATISTA, R. I. T. P.; BALARO, M. F. A.; SARAIVA, H. F. R. de A.; CÔRTES, L. R.; BRANDÃO, F. Z. |
Afiliação: |
VIVIAN ANGÉLICO PEREIRA ALFRADIQUE, Universidade Federal Fluminense (UFF) - Niterói, RJ, Brazil; CAROLINA CERQUEIRA SARMENTO OLIVARES, Universidade Federal Fluminense (UFF) - Niterói, RJ, Brazil; JOANNA MARIA GONÇALVES DE SOUZA-FABJAN, Universidade Federal Fluminense (UFF) - Niterói, RJ, Brazil; JEFERSON FERREIRA DA FONSECA, CNPC; RIBRIO IVAN TAVARES PEREIRA BATISTA, Universidade Federal Fluminense (UFF) - Niterói, RJ, Brazil; MÁRIO FELIPE ALVAREZ BALARO, Universidade Federal Fluminense (UFF) - Niterói, RJ, Brazil; HELENA FABIANA REIS DE ALMEIDA SARAIVA, Universidade Federal Fluminense (UFF) - Niterói, RJ, Brazil; LUANA RANGEL CÔRTES, Universidade Federal Fluminense (UFF) - Niterói, RJ, Brazil; FELIPE ZANDONADI BRANDÃO, Universidade Federal Fluminense (UFF) - Niterói, RJ, Brazil. |
Título: |
Mini-percoll technique induces Similar capacitation features in domestic ruminant frozen-thawed spermatozoa regardless of the presence of heparin. |
Ano de publicação: |
2019 |
Fonte/Imprenta: |
Acta Scientiae Veterinariae, v. 47, Pub. 1707, p. 1-8, 2019. |
DOI: |
10.22456/1679-9216.98317 |
Idioma: |
Inglês |
Conteúdo: |
Background: Sperm capacitation is a process consists of a series of functional, biochemical, and biophysical modifications that render the ejaculated sperm competent for oocyte fertilization. Secreted by the female reproductive tract epithelium, heparin promotes capacitation by binding to and removing seminal plasma proteins, which are adsorbed to the sperm PM and would inhibit capacitation. There is substantial evidence that cryopreservation promotes capacitation-like changes in bull, ram and buck sperm. Our general hypotheses were: (a) cryopreserved ram sperm suffer capacitation more quickly than buck and bull sperm under the same conditions; (b) the capacitation status of ruminant cryopreserved sperm is similar whether or not heparin is present after the mini-Percoll technique; and (c) ruminant frozen-thawed sperm selected by mini-Percoll and incubated within media without heparin supplementation is not impaired in terms of capacitation status and sperm agglutination. This study aimed to compare sperm parameters of ovine, caprine, and bovine frozen-thawed sperm after mini-Percoll processing followed by incubation with or without heparin supplementation. Materials, Methods & Results: Commercial semen of all species were used. Sperm samples were selected by mini-Percoll and supplemented (or not) with heparin within an incubation medium for 18 h. Sperm kinematics (CASA system analyzes), capacitation status (CTC staining) and sperm agglutination were evaluated after thawing, mini-Percoll, 1.5 h, 3 h, 6 h and 18 h. In comparison with post-thawing analysis, ovine species demonstrated a reduction (P < 0.05) in most of the sperm motility parameters after mini-Percoll. Conversely, ovine samples presented the highest (P < 0.05) rate of acrosome-reacted cells after mini-Percoll. Heparin supplementation did not affect most of the parameters evaluated (P > 0.05). In caprine and bovine species, a lower (P < 0.05) rate of sperm agglutination was observed in the presence of heparin at 18 h of incubation. In the absence of heparin, ovine samples showed a higher (P < 0.05) agglutination rate compared to the bovine species after long incubation period. Discussion: The present study compared sperm parameters (sperm kinematics, agglutination rate and capacitation status) of ruminant frozen-thawed sperm after mini-Percoll selection followed by in vitro incubation with or without heparin supplementation. In this study, it was observed the same rate of capacitated cells after the sperm selection (min-Percoll) between ruminant species. This indicate that the capacitation process occurs similarly between ruminant species, refuting the first hypothesis of this study. The presence of heparin did not influence the capacitation status of ruminant frozen-thawed sperm after mini-Percoll selection, it demonstrates that the second hypothesis was supported by this study making more economic and practical the use of ruminant frozen-thawed semen. The absence of heparin in the incubation medium did not harmed the capacitation status and sperm agglutination of ruminant frozen-thawed sperm. This supported the third hypothesis of the current study and indicate that the use of mini-Percoll technique regardless the presence of heparin could be a useful alternative for the preparation of ruminant frozen-thawed sperm. In conclusion, the capacitation status of ruminant frozen-thawed sperm is similar whether or not heparin is present after the mini-Percoll technique. MenosBackground: Sperm capacitation is a process consists of a series of functional, biochemical, and biophysical modifications that render the ejaculated sperm competent for oocyte fertilization. Secreted by the female reproductive tract epithelium, heparin promotes capacitation by binding to and removing seminal plasma proteins, which are adsorbed to the sperm PM and would inhibit capacitation. There is substantial evidence that cryopreservation promotes capacitation-like changes in bull, ram and buck sperm. Our general hypotheses were: (a) cryopreserved ram sperm suffer capacitation more quickly than buck and bull sperm under the same conditions; (b) the capacitation status of ruminant cryopreserved sperm is similar whether or not heparin is present after the mini-Percoll technique; and (c) ruminant frozen-thawed sperm selected by mini-Percoll and incubated within media without heparin supplementation is not impaired in terms of capacitation status and sperm agglutination. This study aimed to compare sperm parameters of ovine, caprine, and bovine frozen-thawed sperm after mini-Percoll processing followed by incubation with or without heparin supplementation. Materials, Methods & Results: Commercial semen of all species were used. Sperm samples were selected by mini-Percoll and supplemented (or not) with heparin within an incubation medium for 18 h. Sperm kinematics (CASA system analyzes), capacitation status (CTC staining) and sperm agglutination were evaluated after thawing, ... Mostrar Tudo |
Palavras-Chave: |
Billygoats; CTC. |
Thesagro: |
Bode; Bovino; Caprino; Carneiro; Espermatozóide; Heparina; Ovino; Reprodução Animal; Sêmen. |
Thesaurus NAL: |
Animal reproduction; Assisted reproductive technologies; Bulls; Cattle; Goats; Heparin; Rams; Sheep; Sperm capacitation. |
Categoria do assunto: |
L Ciência Animal e Produtos de Origem Animal |
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/209450/1/cnpc-2019-Mini-Percoll.pdf
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Marc: |
LEADER 04821naa a2200469 a 4500 001 2119218 005 2020-01-23 008 2019 bl uuuu u00u1 u #d 024 7 $a10.22456/1679-9216.98317$2DOI 100 1 $aALFRADIQUE, V. A. P. 245 $aMini-percoll technique induces Similar capacitation features in domestic ruminant frozen-thawed spermatozoa regardless of the presence of heparin.$h[electronic resource] 260 $c2019 520 $aBackground: Sperm capacitation is a process consists of a series of functional, biochemical, and biophysical modifications that render the ejaculated sperm competent for oocyte fertilization. Secreted by the female reproductive tract epithelium, heparin promotes capacitation by binding to and removing seminal plasma proteins, which are adsorbed to the sperm PM and would inhibit capacitation. There is substantial evidence that cryopreservation promotes capacitation-like changes in bull, ram and buck sperm. Our general hypotheses were: (a) cryopreserved ram sperm suffer capacitation more quickly than buck and bull sperm under the same conditions; (b) the capacitation status of ruminant cryopreserved sperm is similar whether or not heparin is present after the mini-Percoll technique; and (c) ruminant frozen-thawed sperm selected by mini-Percoll and incubated within media without heparin supplementation is not impaired in terms of capacitation status and sperm agglutination. This study aimed to compare sperm parameters of ovine, caprine, and bovine frozen-thawed sperm after mini-Percoll processing followed by incubation with or without heparin supplementation. Materials, Methods & Results: Commercial semen of all species were used. Sperm samples were selected by mini-Percoll and supplemented (or not) with heparin within an incubation medium for 18 h. Sperm kinematics (CASA system analyzes), capacitation status (CTC staining) and sperm agglutination were evaluated after thawing, mini-Percoll, 1.5 h, 3 h, 6 h and 18 h. In comparison with post-thawing analysis, ovine species demonstrated a reduction (P < 0.05) in most of the sperm motility parameters after mini-Percoll. Conversely, ovine samples presented the highest (P < 0.05) rate of acrosome-reacted cells after mini-Percoll. Heparin supplementation did not affect most of the parameters evaluated (P > 0.05). In caprine and bovine species, a lower (P < 0.05) rate of sperm agglutination was observed in the presence of heparin at 18 h of incubation. In the absence of heparin, ovine samples showed a higher (P < 0.05) agglutination rate compared to the bovine species after long incubation period. Discussion: The present study compared sperm parameters (sperm kinematics, agglutination rate and capacitation status) of ruminant frozen-thawed sperm after mini-Percoll selection followed by in vitro incubation with or without heparin supplementation. In this study, it was observed the same rate of capacitated cells after the sperm selection (min-Percoll) between ruminant species. This indicate that the capacitation process occurs similarly between ruminant species, refuting the first hypothesis of this study. The presence of heparin did not influence the capacitation status of ruminant frozen-thawed sperm after mini-Percoll selection, it demonstrates that the second hypothesis was supported by this study making more economic and practical the use of ruminant frozen-thawed semen. The absence of heparin in the incubation medium did not harmed the capacitation status and sperm agglutination of ruminant frozen-thawed sperm. This supported the third hypothesis of the current study and indicate that the use of mini-Percoll technique regardless the presence of heparin could be a useful alternative for the preparation of ruminant frozen-thawed sperm. In conclusion, the capacitation status of ruminant frozen-thawed sperm is similar whether or not heparin is present after the mini-Percoll technique. 650 $aAnimal reproduction 650 $aAssisted reproductive technologies 650 $aBulls 650 $aCattle 650 $aGoats 650 $aHeparin 650 $aRams 650 $aSheep 650 $aSperm capacitation 650 $aBode 650 $aBovino 650 $aCaprino 650 $aCarneiro 650 $aEspermatozóide 650 $aHeparina 650 $aOvino 650 $aReprodução Animal 650 $aSêmen 653 $aBillygoats 653 $aCTC 700 1 $aOLIVARES, C. C. S. 700 1 $aSOUZA-FABJAN, J. M. G. de 700 1 $aFONSECA, J. F. da 700 1 $aBATISTA, R. I. T. P. 700 1 $aBALARO, M. F. A. 700 1 $aSARAIVA, H. F. R. de A. 700 1 $aCÔRTES, L. R. 700 1 $aBRANDÃO, F. Z. 773 $tActa Scientiae Veterinariae$gv. 47, Pub. 1707, p. 1-8, 2019.
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Embrapa Caprinos e Ovinos (CNPC) |
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